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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in lots of tumors, which ends up in chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital position in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two added splice variants ended up claimed but specific nucleotide sequence data just isn't still obtainable.
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Blocking peptides are peptides that bind particularly on the focus on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This guideline outlines even more feasible optimization of mobile and immunoassay parameters to make certain the very best final results are...
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This information outlines more possible optimization of mobile and immunoassay parameters to make certain the very best benefits are...
Antibodies bound to the blocking peptide now here not bind for the epitope on the focus on protein. By evaluating the staining through the blocked antibody versus the antibody by yourself, you can see which staining is certain.
Concentrate on Details Cyclin E belongs into the very conserved cyclin spouse and children, whose associates are characterised by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation designs which add to the temporal coordination of every mitotic celebration. Cyclin E sorts a complex with and functions being a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary and is particularly degraded as cells development through S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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